This application detects fluorescent foci within cellular nuclei and quantifies their count, area and intensity.
In parallel, the intensity of a labeled protein of interest in the nuclei is measured and quantified as well.
Single cell analysis of RNA FISH for cytokine expression
In this application note, we utilized WiScan® Hermes high content microscope to investigate immune responses to stimuli by primary human monocyte-derived macrophages, which exhibit significant variability in gene expression between different cells. WiScan® Hermes High content wide field microscope offers the ability to work with large sample sizes while investigating subcellular features at the single cell level.
Высококачественные автоматизированные решения
для визуализации клеток и анализа изображений
Поля помеченные * - обязательны для заполнения
Поля помеченные * - обязательны для заполнения
Поля помеченные * - обязательны для заполнения