Colony Detection & Cell Count in Colonies

This application infers the number of cells present in a tightly packed colony, where individual nuclei are difficult to identify.

The algorithm quantifies the cell count by identifying two cell populations: single cells and multi-cell colonies.  It then uses the average intensity of a single cell to extrapolate the number of cells contained within a colony

• True cell per colony measurement
• Area and cell density metrics
• Colony shape & morphology extracted
• Compatible with bright field images