Description
| The CytoSinct™ CD4 Nanobeads (human) are used for separating CD4+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension based on the surface expression of human CD4. The surface of Nanobeads are labeled with anti-human CD4 monoclonal antibody. To begin the isolation, Nanobeads are added to the cells. The CD4 antibodies preconjugated to the Nanobeads can bind the target cells expressing CD4 on cell surface. The cells/beads mixed suspension is loaded onto a CytoSinct™ Column which is effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other compatible cell isolation columns and magnets. The Nanobeads-labeled CD4+ cells are retained within the column and enriched during the wash step when Isolation Buffer is used to flush out the CD4- cells. After removing magnetic field, the target CD4+ cells can be easily eluted from the column. |
Note
For research use only. Not for diagnostic use. Not for resale. Genscript will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Properties
| Components | Nanobeads conjugated to monoclonal mouse anti-human CD4 antibodies (isotype: mouse IgG1) under optimal conditions. | ||||||
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| Reactivity | Human | ||||||
| Formulation | Supplied in buffer containing stabilizer | ||||||
| Storage | Store undiluted between 2°C and 8°C. Do not freeze. |
Applications
| Positive selection or depletion of CD4+ T cells from leukapheresis, PBMC or cell cultures. Isolated CD4+ T cells can be used for culture and expansion, flow cytometry, T cells functional assays such as cytokine production cytotoxicity, coculture experiments of CD4+ T cells with dendritic cells or B cells for evaluation of the activation of antigen-presenting cells, and translational research. |
Поля помеченные * - обязательны для заполнения
Поля помеченные * - обязательны для заполнения
Поля помеченные * - обязательны для заполнения
