Description
| The Enceed™ T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed™ T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation. The recommended titer of 1:200 has been found to achieve efficient stimulation of majority of T cell subsets. For special applications, the optimal stimulation titer should be determined experimentally. Excessive amount of the Enceed™ T cell Activation reagent may result in over-activation of T cells risking activation-induced cell death. During cell expansion using the Enceed™ T cell Activation reagent, the cells take up to 13 days after reagent application to show optimal expansion. Restimulation after 13 days can be performed for longer cultivation. The Enceed™ T cell Activation reagent can be used together with cytokines such as human IL-2, IL-15, and IL-7 for more efficient activation. |
Note
For research use only. Not intended for human and animal therapeutic or diagnostic use.
| Reactivity | Human |
| Formulation | Biodegradable matrix-coated nanoparticles conjugated with anti-CD3 and anti-CD28 antibodies supplied in phosphate buffered-saline (PBS), containing Human Serum Albumin (HSA), pH 7.0 — 7.4. |
| Storage | Store at 2-8 °C. Do not freeze. |
Applications
| Enceed™ T cell Activation reagent has been developed for in vitro activation and expansion of enriched T cell populations or cells from hematological cell populations (e.g. PBMCs). |
Поля помеченные * - обязательны для заполнения
Поля помеченные * - обязательны для заполнения
Поля помеченные * - обязательны для заполнения
